Downstream Processing of Monoclonal Antibodies: Current Practices and Future Opportunities

نویسندگان

  • Uwe Gottschalk
  • Brian Kelley
چکیده

s of Papers, 232nd ACS Meeting, San Francisco, CA, 2006. Yigzaw, Y. , Piper, R. , Tran , M. , Shukla , A.A. ( 2006 ). Exploitation of the adsorptive properties of depth filters for host cell protein removal during monoclonal antibody purifi cation. Biotechnology Progress 22, 288–296. Roque, A.C.A. , Silva, C.S.O. , Taipa, M.A. (2007). Affi nity-based methodologies and ligands for antibody purifi cation: Advances and perspectives. Journal of Chromatography A 1160, 44–55. Zhou, J.X., Tressel, T. (2006). Basic concepts in Q membrane chromatography for large-scale antibody production . Biotechnology Progress 22, 341–349. Luan, Y.-T. (2006). Defi ned medium development for high yielding mammalian cell culture processes. BioProcess International Conference & Exhibition: IBC’s 7th International Cell Culture & Upstream Processing, San Francisco, CA, 2006. Jagschies, G., Grönberg, A., Björkman, T., Lacki, K., Johansson, H.J. (2006). Technical and economical evaluation of downstream processing options for monoclonal antibody (Mab) production. BioPharm International 19 (suppl.), 2–8. Kelley, B.D. (2007). Very large scale monoclonal antibody purification: The case for conventional unit operations. Biotechnology Progress 23, 995–1008. Hagel, L., Jagschies, G., Sofer, G. (2007). Production scenarios. In Hagel, L., Jagschies, G., Sofer, G. (eds.) Handbook of Process Chromatography: Development, Manufacturing, Validation and Economics , 2nd eds., Academic Press, London, pp.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Multimodal chromatography: debottlenecking the downstream processing of monoclonal antibodies

2015 The commercial potential of monoclonal antibodies (mAbs) has been continuously increasing during the last years alongside with the number of approved mAb-based drugs and clinical trials. Despite their effectiveness and safety, the general access to this class of biopharmaceuticals is barred by high selling prices. Downstream processing is now considered the bottleneck in the manufacturing ...

متن کامل

A Single-use Strategy to Enable Manufacturing of Affordable Biologics

The current processing paradigm of large manufacturing facilities dedicated to single product production is no longer an effective approach for best manufacturing practices. Increasing competition for new indications and the launch of biosimilars for the monoclonal antibody market have put pressure on manufacturers to produce at lower cost. Single-use technologies and continuous upstream proces...

متن کامل

Monoclonal Antibodies

SUMMARY Monoclonal Antibodies are identical antibodies and Can be generated in ultimated amounts by countinuos cultures of single antibody- secreting cells. These cell lines are produced by cell fusion of normal lymphocytes to cells of a myeloma tumor cell line, which confers on the antibody producing hybrid cell, immortality and the ability grow as atumor in animals. monoclonal antibodies c...

متن کامل

Production and Characterization of Murine Monoclonal Antibodies to Leishmania Gp63 Antigen

Background : Production of monoclonal antibodies to Leishmania antigens assists the identification and characterization of these organisms. Objective: Production of monoclonal antibodies against epitopes on the gp63. Methods: Two murine monoclonal antibodies to gp63 were produced and characterized. The reactions of both antibodies with soluble leishmanial antigens, purified gp63 and truncated r...

متن کامل

Novel downstream processes for the purification of monoclonal antibodies based on aqueous two phase partitioning and hydrophobic interaction chromatography

Monoclonal antibodies have proven their potential as therapeutic molecules with applications as diverse as diagnosis, oncology and treatment of autoimmune diseases; the need for their supply is higher than it ever was, with the global market for monoclonal antibodies being expected to grow to nearly $58 billion in 2016. The main challenge for this growth remains the fact these products need to ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2008